Following reverse transcription with integrated template switching, all individually tagged cDNAs can be combined, which enables all subsequent library construction steps to be performed in a single tube – saving significant time and library prep costs. The QIAseq UPX 3' Transcriptome Kit presents a unique advantage where each cell is tagged with a unique ID (up to 384 different IDs) and each RNA molecule is tagged with a Unique Molecular Index (UMI) during reverse transcription.Use 1 nM RNA-seq libraries as input for the denaturation procedure to ultimately load 3 pM for the MiSeq (V3 kit) and 1.2 pM for the NextSeq. 333314), which contains laboratory-verified forward and reverse primers together with a DNA standard, is highly recommended for accurate quantification of the prepared library. As a result, QIAGEN’s QIAseq Library Quant Array Kit (cat. Real-time PCR-based methods provide an accurate quantification of complete RNA-seq libraries with full adapter sequences. Library QC using an Agilent Bioanalyzer is shown with a peak at 425 bp. A portion of the 11 μl sequencing library was used as the starting material for the library QC and quantification.Want to try this solution for the first time? Request a quote for a trial kit. All reagents necessary for starting with cells (such as lysis reagents) or RNA are included in the kit. After cDNA synthesis, combine each plate into a single tube to complete the library preparation. To build libraries for RNA-seq, simply add 1 to 100 cells into each well or 10 pg to 1 ng of RNA to reconstitute a reverse transcription reaction. Each kit is enough for 96 or 384 libraries, with kit format comprising of either a 96-well multi-break plates or 384-well single use plates with reverse transcription primers already lyophilized in the bottom of the plate for ease of use. QIAseq UPX 3' Transcriptome Kits enable high-throughput gene expression analysis from single cells and previously isolated RNA. Includes cloud-based read alignment and single-cell or low-input analysis.UPX tagging allows 4608–18,432 samples per single sequencing lane.UMIs eliminate library amplification bias for accurate gene expression.LNA-enhanced chemistry for increased accuracy, specificity and sensitivity.Start with 1–100 cells or 10 pg to 1 ng of isolated RNA.For high-throughput 3' transcriptome NGS from ultralow amounts of RNA
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